A Review Of flow cytometers
A Review Of flow cytometers
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Data-enriched characterization of cell populations. Morphological information from photographs can insert for the richness of flow cytometry data. One example is, the figure displays an or else traditional apoptosis assay working with Annexin V and PI, including mobile imaging to characterize cells in Just about every inhabitants to reveal morphologically distinctive attributes. These insights could not are acquired from multiplex staining alone.
As samples are acquired around the Attune CytPix Flow Cytometer, the large-velocity brightfield digital camera captures and outlets photographs of detected activities at a charge nearly 6,000 photos for each next, based on the flow fee and image sizing. For increased flexibility, Attune Cytometric Application helps you to change the image capture frequency as wanted and choose a specific gate for impression capture, if demanded.
(B) RAW 264.7 macrophages will often be annotated inaccurately with respect to particle depend because of their complexity and noncircular borders. The volume of singlets obtainable for Examination will increase as soon as the person trains and employs a far more correct model.
Find out new insights into your experimental data by visualizing populations using blended fluorescence and morphology data. Our application interface wizards help downsampling, concatenation (merged documents) and dimensionality equipment which are built to the program for an intuitive, streamlined information Examination knowledge.
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Right here, a highly trained consumer has gated singlets confidently. Right after evaluating the manual singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate contains greater than 4% aggregates.
Pair your Attune flow cytometer having an autosampler for amplified workflow efficiency, and incorporate robotic integration for max throughput.
Steady impression excellent even at superior throughput Acoustic focusing as well as a higher-velocity digicam Blend to graphic these T cells regularly at minimal or substantial flow prices. Simply change concentration and camera settings to meet experimental demands.
The Attune NxT cytometer may be the reputable workhorse instrument your lab can rely on for consistent and trusted overall performance. The Attune CytPix product incorporates a high-speed brightfield digicam and AI-pushed graphic Examination program, with modern upgrades including equipment Discovering instruments and an intuitive consumer interface, enabling enhanced information precision, accuracy, and faster insights into sample biology.
End users can look at function pictures in real-time to confirm sample high-quality, optimize protocols, and record the morphology of each mobile population determined for Examination. Imaging seize also Gains from acoustic concentrating, which helps you to placement the cells to ensure a sharp, centered image is obtained.
Seem waves help specific cell alignment, leading to Fantastic knowledge excellent and ~10X speedier sample flow fees.
We Beforehand shown the power of imaging Vehicle-T/Ramos mobile interactions. Let’s evaluate just the inhabitants of biggest fascination, the double good functions, To find out more. We can now use prolonged morphological parameters (in this article, circularity and skewness of depth) to further look at the attributes of these populations and refine gating on these events, raising information robustness.
Leverage the power of Superior technologies and innovation to accelerate your discoveries. Trust while in the Attune Flow Cytometer household to deliver the reliability, speed, and precision that established your get the job done aside.
Flexible payment prepare from Agilent Money Solutions permit you to get the critical instrumentation you'll need for speedy project, or to expand your lab.
Gating on these prolonged parameters lets you rapidly and precisely discover populations of fascination and ensure gating method with little if any guide evaluation. The Attune computer software provides access to point out-of-the-artwork equipment get more info learning Evaluation resources inside of an expertly designed consumer interface, rendering it extremely simple to use.
The Attune Xenith cytometer is really a higher-parameter spectral analyzer, giving both equally spectral unmixing and conventional flow capabilities for in depth and adaptable mobile Investigation.
Agilent Price Assure assures you at the least a decade of instrument use through the day you purchase. Usually, We are going to credit history you with the residual value of the technique towards an upgraded design.
What appeared to be a homogeneous inhabitants is definitely much more varied—an insight that needs to be regarded as when interpreting final results.